TLE1 Antibody / Transducin-like enhancer protein 1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q04724

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 175-338 from the human protein was used as the immunogen for the TLE1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

TLEP1-1

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TLE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TLE1 antibody is available for research use only.

Storage Conditions

Store the TLE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the TLE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.