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TCF4 Antibody

Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15884

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 365-671 from the human protein was used as the immunogen for this TCF4 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

TCF4/1705

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TCF4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TCF4 antibody is available for research use only.

Storage Conditions

Store the TCF4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TCF4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with TCF4 antibody (clone TCF4/1705) . Predicted molecular weight ~71 kDa.

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