TCF4 Antibody
Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P15884
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 365-671 from the human protein was used as the immunogen for this TCF4 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
TCF4/1705
Applications
ELISA, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TCF4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TCF4 antibody is available for research use only.
Storage Conditions
Store the TCF4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the TCF4 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with TCF4 antibody (clone TCF4/1705) . Predicted molecular weight ~71 kDa.
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