Spectrin alpha 1 Antibody / SPTA1

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous and chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin Ia and spectrin Ib are present in erythrocytes, whereas spectrin IIa (also designated fodrin alpha) and spectrin IIb (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P02549

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 356-475 from the human protein was used as the immunogen for the Spectrin alpha 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SPTA1/1832

Applications

ELISA, WB. IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Spectrin alpha 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Spectrin alpha 1 antibody is available for research use only.

Storage Conditions

Store the Spectrin alpha 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Spectrin alpha 1 antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface

Image Legend

IHC staining of FFPE human tonsil with Spectrin alpha 1 antibody (clone SPTA1/1832) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.