PU.1 Antibody / SPI1

Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P17947

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TFPU1-1

Applications

IHC-P, WB, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PU.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PU.1 antibody is available for research use only.

Storage Conditions

Store the PU.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone TFPU1-1) . Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.