SOX4 Antibody

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-ÃŽÂ²-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival in vitro and for primary tumor growth and metastasis in vivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q06945

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the SOX4 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SOX4/2540

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the SOX4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This SOX4 antibody is available for research use only.

Storage Conditions

Store the SOX4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the SOX4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human colon carcinoma with SOX4 antibody (clone SOX4/2540) . HIER: boil tissue sections in pH6, 10mM citrate buffer for 10-20 min and allow to cool before testing.