What is the catalog number for Ki67 Antibody?

The catalog number for Ki67 Antibody is V3871SAF-100UG.

What sizes are available for Ki67 Antibody?

Ki67 Antibody is available in the following size(s): 100 µg.

Is Ki67 Antibody in stock?

Ki67 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Ki67 Antibody

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P46013

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PMKI67-1

Applications

IHC-P, FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Ki67 antibody is available for research use only.

Storage Conditions

Store the Ki67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1) . Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.