MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase
Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase) . It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q13126
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 97-196 from the human protein was used as the immunogen for the MTAP antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
MTAP/1813
Applications
ELISA, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MTAP antibody is available for research use only.
Storage Conditions
Store the MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the MTAP antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human A431 cell lysate with MTAP antibody (clone MTAP/1813) . Expected molecular weight: 26-38 kDa (multiple isoforms) .
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