Glial Fibrillary Acidic Protein Antibody / GFAP
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P14136
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 101-200 were used as the immunogen for the Glial Fibrillary Acidic Protein antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GFAP/2076
Applications
FACS, IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Glial Fibrillary Acidic Protein antibody is available for research use only.
Storage Conditions
Store the Glial Fibrillary Acidic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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