Glial Fibrillary Acidic Protein Antibody / GFAP

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P14136

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 101-200 were used as the immunogen for the Glial Fibrillary Acidic Protein antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

GFAP/2076

Applications

FACS, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Glial Fibrillary Acidic Protein antibody is available for research use only.

Storage Conditions

Store the Glial Fibrillary Acidic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Flow cytometry testing of fixed human T98G cells with Glial Fibrillary Acidic Protein antibody (clone GFAP/2076) ; Red=isotype control, Blue= Glial Fibrillary Acidic Protein antibody.