KRT6 Antibody / Cytokeratin 6

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P02538

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human KRT6A protein was used as the immunogen for the KRT6 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CTKN6-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the KRT6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This KRT6 antibody is available for research use only.

Storage Conditions

Store the KRT6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the KRT6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human basal cell carcinoma with KRT6 antibody (clone CTKN6-1) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.