Filaggrin Antibody

Filaggrin (FLG) aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P20930

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 998-1104 from the human protein were used as the immunogen for this Filaggrin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FLG1-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Filaggrin antibody is available for research use only.

Storage Conditions

Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Filaggrin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with Filaggrin antibody (clone FLG1-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.