CD11c Antibody

Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs) . A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format)

UniProt

P20702

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein (aa 637-827) was used as the immunogen for the CD11c antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ITGAX/2507

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD11c antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD11c antibody is available for research use only.

Storage Conditions

Store the CD11c antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD11c antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with CD11c antibody (clone ITGAX/2507) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.