GAD67 Antibody / GAD1
This mAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1) . There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1) . GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q99259
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
GAD1/2391
Applications
ELISA, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the GAD67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This GAD67 antibody is available for research use only.
Storage Conditions
Store the GAD67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the GAD67 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human brain lysate with GAD67 antibody. Expected molecular weight ~67 kDa.
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