Recombinant Anti-Kappa Light Chain Antibody

This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01601, P01834

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Ig kappa chain was used as the immunogen for this recombinant anti-Kappa light chain antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RKLC709

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant anti-Kappa light chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant anti-Kappa light chain antibody is available for research use only.

Storage Conditions

Store the recombinant anti-Kappa light chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the recombinant anti-Kappa light chain antibody for each application should be determined by the researcher.

Location

Cell Surface, cytoplasmic and secreted

Image Legend

IHC testing of FFPE human tonsil tissue with recombinant anti-Kappa light chain antibody (clone rKLC709) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.