Estrogen Receptor alpha Antibody

This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P03372

Host

Mouse

Reactivity

Human

Immunogen

Full length human protein was used as the immunogen for this Estrogen Receptor alpha antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

ESR1/1935

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Estrogen Receptor alpha antibody is available for research use only.

Storage Conditions

Store the Estrogen Receptor alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the Estrogen Receptor alpha antibody for each application should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of human endometrial carcinoma stained with Estrogen Receptor alpha antibody (clone ESR1/1935) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.