Filaggrin Antibody
Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P20930
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 998-1104 from the human protein were used as the immunogen for this Filaggrin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
FLG/1945
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Filaggrin antibody is available for research use only.
Storage Conditions
Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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