HER2 Antibody / ErbB2
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL
UniProt
P04626
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 311-462 from the human protein was used as the immunogen for this HER2 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
ERBB2/2452
Applications
IHC-P, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HER2 antibody is available for research use only.
Storage Conditions
Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Location
Cell membrane (This mAb binds to the extracellular/cell surface region of the protein)
Image Legend
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/2452, green) and Reddot nuclear stain (red) .
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