CD103 Antibody / Integrin alpha E

This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P38570

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ITGAE/2474

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD103 antibody is available for research use only.

Storage Conditions

Store the CD103 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the CD103 antibody may be required for optimal performance.

Location

Cell membrane, cytoplasm

Image Legend

IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2474) . HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.