Elastin Antibody

Elastin is a polymeric protein and is the main component of the extracellular matrix of arteries. It is synthesized and secreted as a soluble, single-chain protein (tropoelastin), which undergoes a number of post-translational modifications prior to its organization into an elastic fiber in the extracellular space. Elastin performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. It imparts elasticity to the connective tissue. During aging, the elasticity of connective tissue is reduced because of the cross-linking of collagenous fibers with elastin. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P15502

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full length human protein was used as the immunogen for the Elastin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ELN/1981

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Elastin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Elastin antibody is available for research use only.

Storage Conditions

Store the Elastin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the Elastin antibody may be required for optimal performance.

Location

Secreted

Image Legend

IHC testing of FFPE human small intestine with Elastin antibody (clone ELN/1981) . Required HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.