IBA1 Antibody / AIF1

AIF1 (Allograft inflammatory factor 1), also called IBA1 (Ionized calcium-binding adapter molecule 1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1/IBA1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, it colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding the protein resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P55008

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 1-146 from the human protein was used as the immunogen for the IBA1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AIF1/1909

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the IBA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This IBA1 antibody is available for research use only.

Storage Conditions

Store the IBA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the IBA1 antibody may be required for optimal performance.

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human lymph node with IBA1 antibody (clone AIF1/1909) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.