Mesothelin Antibody / MSLN

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

Q13421

Host

Mouse

Reactivity

Human, Mouse and Rat

Immunogen

A portion of amino acids 273-407 from the human protein was used as the immunogen for the Mesothelin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MSLN/2131

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Mesothelin antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This Mesothelin antibody is available for research use only.

Storage Conditions

Store the Mesothelin antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Mesothelin antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC staining of FFPE human endometrial carcinoma with biotinylated Mesothelin antibody (clone MSLN/2131) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.