Mesothelin Antibody / MSLN

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

Q13421

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 273-407 from the human protein was used as the immunogen for this Mesothelin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MSLN/2131

Applications

IHC-P, WB

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

After reconstitution, the Mesothelin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Mesothelin antibody is available for research use only.

Storage Conditions

After reconstitution, the Mesothelin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MSLN antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC testing of FFPE human lung mesothelioma with Mesothelin antibody (clone MSLN/2131) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.