Recombinant Basic Cytokeratin Antibody / Rabbit Monoclonal
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Product Specifications
CAS Number
9000-83-3
Specifications
UniProt
Q01546
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Solubilized keratin extract from human stratum corneum was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
KRTH/2147R
Type
Recombinant
Applications
FACS, IF, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Basic Cytokeratin antibody is available for research use only.
Storage Conditions
Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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