ARF1 Antibody / ADP-ribosylation factor 1
The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF s share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P84077
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
The C-terminal amino acid sequence SNQLRNQ from the human protein was used as the immunogen for the ARF1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
1A9/5
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ARF1 antibody is available for research use only.
Storage Conditions
Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Titering of the ARF1 antibody may be required for optimal performance.
Location
Cytoplasmic (Golgi)
Image Legend
Western blot testing of human HEK293 and HepG2 cell lysate with ARF1 antibody (clone 1A9/5) . Predicted molecular weight ~21 kDa.
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