P14ARF Antibody / CDKN2A

The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks) . Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the production of the p19ARF protein in mice and the p14ARF protein in humans. p14ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P42771

Host

Mouse

Reactivity

Human, Rat

Immunogen

Full-length recombinant human protein used as the immunogen for the p14ARF antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

4C6/4

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the p14ARF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This p14ARF antibody is available for research use only.

Storage Conditions

Store the p14ARF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the p14ARF antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human brain with p14ARF antibody (clone 4C6/4) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.