P21 Antibody / p21WAF1
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL, Immunofluorescence: 1-3 µg/mL
UniProt
P38936
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the p21 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
AC8
Applications
WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p21 antibody is available for research use only.
Storage Conditions
Store the p21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the p21 antibody to be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human HepG2 cell lysate with p21 antibody (clone AC8) .
Frequently Asked Questions
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