CD68 Antibody

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, ELISA: order BSA/sodium azide-free format for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P34810

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 150-301 from the human protein was used as the immunogen for the CD68 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

LAMP4/1830

Applications

FACS, ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD68 antibody is available for research use only.

Storage Conditions

Store the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD68 antibody should be determined by the researcher.

Location

Cytoplasmic, membrane

Image Legend

IHC staining of FFPE human tonsil with CD68 antibody (LAMP4/1830) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.