AKT1 Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P31749

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

RASTPK-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This AKT1 antibody is available for research use only.

Storage Conditions

Store the AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nucleus, plasma membrane, cytoplasm

Image Legend

IHC testing of FFPE human pancreas with AKT1 antibody (clone RASTPK-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.