CD21 Antibody

CD21/CR2 is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. This mAb is highly specific to CR2 and shows no cross-reaction with CR1.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P20023

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 44-196 were used as the immunogen for the CD21 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA21-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD21 antibody is available for research use only.

Storage Conditions

Store the CD21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CD21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface

Image Legend

IHC testing of FFPE human tonsil tissue with CD21 antibody (clone CDLA21-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.