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Recombinant Calponin Antibody

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P51911

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCNN1/832

Type

Recombinant

Applications

WB, FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Calponin antibody is available for research use only.

Storage Conditions

Store the recombinant Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone rCNN1/832) . Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.

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