CD44v3 Antibody

This antibody recognizes an epitope encoded by exon v3 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16070

Host

Mouse

Reactivity

Human

Immunogen

An amino acid sequence from the variant 3 domain of CD44 was used as the immunogen for the CD44v3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

3G5

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD44v3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD44v3 antibody is available for research use only.

Storage Conditions

Store the CD44v3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the CD44v3 antibody may be required for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human cervical carcinoma with CD44v3 antibody (clone 3G5) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA buffer for 10-20 min and allow to cool before testing.