Recombinant Chromogranin A Antibody

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P10645

Host

Mouse

Reactivity

Human, Rat

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant Chromogranin A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RCHGA/798

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Chromogranin A antibody is available for research use only.

Storage Conditions

Store the recombinant Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.

Location

Finely granular cytoplasmic

Image Legend

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone rCHGA/798) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.