CD14 Antibody (Macrophage Marker)
Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08571
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant partial protein (within amino acids 25-148) was used as immunogen for this CD14 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LPSR/2386
Applications
WB, FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
CD14 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD14 antibody is available for research use only.
Storage Conditions
CD14 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.
Location
Cell surface, Secreted, Cytoplasmic (Golgi)
Image Legend
Flow cytometry testing of human A549 cells with CD14 antibody (clone LPSR/2386) ; Red=isotype control, Blue= CD14 antibody.
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