CD3 epsilon Antibody

This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL

UniProt

P07766

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 23-119 were used as the immunogen for the CD3 epsilon antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDLA3e-1

Applications

FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CD3 epsilon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD3 epsilon antibody is available for research use only.

Storage Conditions

Store the CD3 epsilon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.