CD14 Antibody (Macrophage Marker)

Recognizes a protein of 55kDa, identified as Cluster of Differentiation 14 (also known lipopolysaccharide receptor or LPSR) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. It is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P08571

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (within amino acids 25-148) was used as immunogen for this CD14 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LPSR/2385

Applications

WB, FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

CD14 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This CD14 antibody is available for research use only.

Storage Conditions

CD14 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Variations in protocols, secondaries and substrates may require the CD14 antibody to be titered up or down for optimal performance.

Location

Cell surface, Secreted, Cytoplasmic (Golgi)

Image Legend

IHC testing of FFPE human lymph node with CD14 antibody (clone LPSR/2385) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.