CD3e Antibody
This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
Specifications
Flow cytometry: 1-2ug/10^6 cells, ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 23-119 were used as the immunogen for the CD3e antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C3e/2478
Applications
FACS, ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD3e antibody is available for research use only.
Storage Conditions
Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd3e-antibody-c3e2478-v3751
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3e antibody should be determined by the researcher.
CAS Number
9007-83-4
Location
Cell surface and cytoplasmic
Image Legend
Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/2478) ; Red=isotype control, Blue= CD3e antibody.
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