CD3e Antibody
This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 23-119 were used as the immunogen for the CD3e antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C3e/2478
Applications
FACS, ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD3e antibody is available for research use only.
Storage Conditions
Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3e antibody should be determined by the researcher.
Location
Cell surface and cytoplasmic
Image Legend
Flow cytometry testing of human Jurkat cells with CD3e antibody (clone C3e/2478) ; Red=isotype control, Blue= CD3e antibody.
Documents
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