Recombinant CD99 Antibody / Rabbit Monoclonal

CD99 is found on the cell surface of Ewing's sarcoma tumors and is positive in granulosa cell tumors. It is more expressed in malignant gliomas than in the brain, and such overexpression results in higher levels of invasiveness and lower rates of survival. Antibodies to CD99 are used in diagnostic immunohistochemistry to distinguish Ewing's sarcoma from other tumours of similar histological appearance, as well as for the identification of thymic tumours, and of spindle cell tumours, such as synovial sarcoma, haemangiopericytoma, and meningioma.[Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P14209

Host

Rabbit

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant CD99 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDLA99-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD99 antibody is available for research use only.

Storage Conditions

Store the recombinant CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface

Image Legend

IHC testing of FFPE human Ewing's sarcoma with recombinant CD99 antibody (clone CDLA99-2R) . Required HIER: steam sections in 10mM Citrate buffer, pH 6.0, for 10-20 min.