CDC2 Antibody / CDK1

Recognizes a 34kDa protein (cdk1), identified as Cdk1/Cdc2/p34cdc2 (a catalytic subunit of Maturation Promoting Factor) . Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2) . It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This mAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Product Specifications

CAS Number

9007-83-4

Specifications

Inhibits activation of p34cdc2 kinase by cyclins, Immunofluorescence: 1-2 µg/mL, Immunoprecipitation, Kinase assay, Western blot, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

UniProt

P06493

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A partial protein (the C-terminal two-thirds of Xenopus CDC2) was used as the immunogen for this CDC2 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

A17.1.1

Applications

Inhibition, IF, IP, Kinase assay, WB, IHC-P

Purity

Protein G purified antibody

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDC2 antibody is available for research use only.

Storage Conditions

Store the CDC2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear and Cytoplasmic

Image Legend

IHC staining of FFPE human tonsil with CDC2 antibody (clone A17.1.1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.