What is the catalog number for Recombinant MUC-1 Antibody?

The catalog number for Recombinant MUC-1 Antibody is V3744IHC-7ML.

What sizes are available for Recombinant MUC-1 Antibody?

Recombinant MUC-1 Antibody is available in the following size(s): 7 mL.

Is Recombinant MUC-1 Antibody in stock?

Recombinant MUC-1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant MUC-1 Antibody

This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P15941

Host

Mouse

Reactivity

Human

Immunogen

Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RMUC1/960

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant MUC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MUC-1 antibody is available for research use only.

Storage Conditions

Store the recombinant MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant MUC-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic and cell surface

Image Legend

IHC testing of FFPE human ovarian carcinoma with recombinant MUC-1 antibody (clone rMUC1/960) . Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.