CYP2E1 Antibody / Cytochrome P450

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL for 30 min at RT

UniProt

P05181

Host

Mouse

Reactivity

Human

Immunogen

The C-terminal amino acid sequence HIGFGCIPPR was used as the immunogen for the CYP2E1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

M12P4H2

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the CYP2E1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CYP2E1 antibody is available for research use only.

Storage Conditions

Store the CYP2E1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic (ER)

Image Legend

IHC testing of FFPE human hepatic carcinoma with TdT antibody (clone M12P4H2) . Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.