Annexin A1 Antibody

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P04083

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human full-length protein was used as the immunogen for the Annexin A1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

6E4/3

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Annexin A1 antibody is available for research use only.

Storage Conditions

Store the Annexin A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Nuclear and Cytoplasmic

Image Legend

IHC testing of FFPE human bladder carinoma with Annexin A1 antibody (clone 6E4/3) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.