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Recombinant Multi Cytokeratin Antibody / Rabbit Monoclonal

Selected Variant

V3718-100UG

Size: 100 µg

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4) ; 58kDa (CK5) ; 56kDa (CK6) ; 52kDa (CK8) ; 56.5kDa (CK10) ; 53kDa (CK13) and 45kDa (CK18) . This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Host

Rabbit

Reactivity

Human

Immunogen

Human epithelial cell keratin was used as the immunogen for the recombinant Multi Cytokeratin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CTKNM-1R

Type

Recombinant

Applications

IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Multi Cytokeratin antibody is available for research use only.

Storage Conditions

Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skin with recombinant Multi Cytokeratin antibody (clone CTKNM-1R) . HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

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