Recombinant Cytokeratin 7 Antibody

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7) . Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P08729

Host

Mouse

Reactivity

Human

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

ROV-TL12/30

Type

Recombinant

Applications

WB, FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Cytokeratin 7 antibody is available for research use only.

Storage Conditions

Store the recombinant Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human endometrial carcinoma with recombinant Cytokeratin 7 antibody (clone rOV-TL12/30) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.