Recombinant GFAP Antibody / Rabbit Monoclonal

Glial fibrillary acidic protein is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [RefSeq]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P14136

Host

Rabbit

Reactivity

Human

Immunogen

Pig spinal cord GFAP was used as the immunogen for this recombinant GFAP antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GFA12-4R

Type

Recombinant

Applications

IHC-P, IF

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant GFAP antibody is available for research use only.

Storage Conditions

Store the recombinant GFAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone GFA12-4R) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.