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Recombinant E-Cadherin Antibody / Rabbit Monoclonal

Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ECD1-3R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the recombinant E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant E-Cadherin antibody is available for research use only.

Storage Conditions

Store the recombinant E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic, membranous

Image Legend

Formalin-fixed, paraffin-embedded human cervix stained with recombinant E-Cadherin antibody (clone ECD1-3R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

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