Recombinant CD8 Antibody (alpha chain) / Rabbit Monoclonal

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Prediluted IHC only format : incubate for 30 min at RT (1)

UniProt

P01732

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDLA8a-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD8 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This recombinant CD8 antibody is available for research use only.

Storage Conditions

Store the recombinant CD8 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface

Image Legend

IHC testing of FFPE human tonsil with recombinant CD8 antibody (clone CDLA8a-2R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.