Recombinant MUC1 Antibody / Rabbit Monoclonal

Mucin-1/MUC1 protein is encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Family members are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. MUC1 is a transmembrane protein normally expressed on the apical borders of secretory epithelial cells. Overexpression is often associated with colon, breast, ovarian, lung and pancreatic cancers. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P15941

Host

Rabbit

Reactivity

Human

Immunogen

Human milk fat globule membranes were used as the immunogen for this recombinant MUC1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MCN01-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant MUC1 antibody is available for research use only.

Storage Conditions

Store the recombinant MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic and cell surface

Image Legend

IHC testing of FFPE human breast carcinoma with recombinant MUC1 antibody (clone MCN01-2R) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.