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Recombinant S100B Antibody / Rabbit Monoclonal

S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. [Wiki]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P04271

Host

Rabbit

Reactivity

Human

Immunogen

Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PS1B1-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant S100B antibody is available for research use only.

Storage Conditions

Store the recombinant S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human melanoma tissue with recombinant S100B antibody (clone PS1B1-2R) . HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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